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Site-directed mutagenesis was performed using PrimeStar Max (Takara) to generate FLAGHAOTUD5_C224R, FLAGTRIP12_C1959A, FLAGARMC7, HAARMC7, and FLAGZGPAT

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A recent example of promising research in this area is a genetic mouse model that closely recapitulates ICI myocarditis and can be used to investigate therapeutic interventions 14

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